What is the most common and feasible mouse CCR2 inhibitor used?

I need to block the migration of macrophages into brain following a TMEV, virus infection in mice. The best way to follow is to use the mouse CCR2 inhibitors. Please suggest the most common and...

06 July 2018 6,592 0 View

Can anybody share the protocol for the intracellular staining of mouse macrophages from brain for the anaysis of TNF-a production by Flow cytometry?

I need to analyze the TNF-a production from mouse macrophages from brain by Flow cytometry. I see there are different protocols used by people. All I see is the addition of Brefeldin A to the...

05 June 2018 1,343 6 View

What are the suitable markers for activated microglia in mice brain by Flow cytometry?

What are the suitable markers for distinguishing activated macrophages from activated microglia in mice brains by Flow cytometry?

04 May 2018 4,402 0 View

Which splenic DC subset is more specific for CD4+ T cell activation?

I want to set up the co-culture experiment using OT-II CD+T cells and splenic DC subsets for checking the CD4+ T cell activation. I was wondering out of the various splenic DC subsets, which DC...

06 July 2016 9,655 2 View

What are the best markers to differentiate between naive and activated CD8+ T cells in mice?

I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T...

06 July 2016 935 4 View

Which is the best marker to sort splenic macrophages from mice by flow cytometry?

05 June 2016 1,756 3 View

Is it a good idea to detect the good amount of IFN-gamma from the in vitro culture of MTB infected splenocytes?

I want to know whether it is a good idea to measure the IFN-g release by intracellular staining (Flow cytometry) from the in vitro culture of naive splenocytes infected with a specific M....

10 November 2015 9,595 9 View

Is there any other specific technique to identify microglia from mice brain other than Flow cytometry?

I usually look at microglia isolated from mice brain using the standard percol gradient method without using Collagenase/DNAse steps i.e. just meshing the brain with 10 ml syringe plunger and then...

10 November 2015 7,446 6 View

Has anybody used Zombie aqua dye (fixable viability dye for FACS) and can give me an idea about the gating of the cells?

05 June 2015 7,393 5 View

Is CFSE more sensitive method than MTT assay to detect proliferation of cells?

I am doing mixed lymphocyte reaction for testing the reaction of C57BL/6 splenocytes to Balb/c splenocytes. I cultured the whole splenocytes of Balb/c mice with the irradiated splenocytes of...

04 May 2015 3,424 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View