What is the most common and feasible mouse CCR2 inhibitor used?

I need to block the migration of macrophages into brain following a TMEV, virus infection in mice. The best way to follow is to use the mouse CCR2 inhibitors. Please suggest the most common and...

06 July 2018 6,592 0 View

Can anybody share the protocol for the intracellular staining of mouse macrophages from brain for the anaysis of TNF-a production by Flow cytometry?

I need to analyze the TNF-a production from mouse macrophages from brain by Flow cytometry. I see there are different protocols used by people. All I see is the addition of Brefeldin A to the...

05 June 2018 1,343 6 View

What are the suitable markers for activated microglia in mice brain by Flow cytometry?

What are the suitable markers for distinguishing activated macrophages from activated microglia in mice brains by Flow cytometry?

04 May 2018 4,402 0 View

Which splenic DC subset is more specific for CD4+ T cell activation?

I want to set up the co-culture experiment using OT-II CD+T cells and splenic DC subsets for checking the CD4+ T cell activation. I was wondering out of the various splenic DC subsets, which DC...

06 July 2016 9,655 2 View

Which is the best marker to sort splenic macrophages from mice by flow cytometry?

05 June 2016 1,756 3 View

Which is the best antibody to be used for sorting splenic DCs by FACS?

I have to sort splenic DCs from mice and was wondering which antibody is best to sort splenic DCs. Can I use just mouse anti- CD11c antibody for sorting splenic DCs as CD11c+ cells. Also, how many...

03 April 2016 3,397 4 View

Is it a good idea to detect the good amount of IFN-gamma from the in vitro culture of MTB infected splenocytes?

I want to know whether it is a good idea to measure the IFN-g release by intracellular staining (Flow cytometry) from the in vitro culture of naive splenocytes infected with a specific M....

10 November 2015 9,595 9 View

Is there any other specific technique to identify microglia from mice brain other than Flow cytometry?

I usually look at microglia isolated from mice brain using the standard percol gradient method without using Collagenase/DNAse steps i.e. just meshing the brain with 10 ml syringe plunger and then...

10 November 2015 7,446 6 View

Has anybody used Zombie aqua dye (fixable viability dye for FACS) and can give me an idea about the gating of the cells?

05 June 2015 7,393 5 View

Is CFSE more sensitive method than MTT assay to detect proliferation of cells?

I am doing mixed lymphocyte reaction for testing the reaction of C57BL/6 splenocytes to Balb/c splenocytes. I cultured the whole splenocytes of Balb/c mice with the irradiated splenocytes of...

04 May 2015 3,424 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View