What are the suitable markers for distinguishing activated macrophages from activated microglia in mice brains by Flow cytometry?
I need to block the migration of macrophages into brain following a TMEV, virus infection in mice. The best way to follow is to use the mouse CCR2 inhibitors. Please suggest the most common and...
06 July 2018 6,722 0 View
I need to analyze the TNF-a production from mouse macrophages from brain by Flow cytometry. I see there are different protocols used by people. All I see is the addition of Brefeldin A to the...
05 June 2018 1,456 6 View
I want to set up the co-culture experiment using OT-II CD+T cells and splenic DC subsets for checking the CD4+ T cell activation. I was wondering out of the various splenic DC subsets, which DC...
06 July 2016 9,770 2 View
I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T...
06 July 2016 1,048 4 View
I wanted to know which marker I should use to sort the splenic macrophages from mice by flow cytometry? Actually I checked the literature and observed that people use CD11b or F4/80 as the marker...
05 June 2016 1,873 3 View
I have to sort splenic DCs from mice and was wondering which antibody is best to sort splenic DCs. Can I use just mouse anti- CD11c antibody for sorting splenic DCs as CD11c+ cells. Also, how many...
03 April 2016 3,501 4 View
I want to know whether it is a good idea to measure the IFN-g release by intracellular staining (Flow cytometry) from the in vitro culture of naive splenocytes infected with a specific M....
10 November 2015 9,697 9 View
I usually look at microglia isolated from mice brain using the standard percol gradient method without using Collagenase/DNAse steps i.e. just meshing the brain with 10 ml syringe plunger and then...
10 November 2015 7,578 6 View
I used Zombie aqua dye (fixable viability dye) for the first time to stain splenocytes by Flow cytometry. I mixed the live cells with heat killed cells (heated at 65 degrees for 1 min) in 1:1...
05 June 2015 7,530 5 View
I am doing mixed lymphocyte reaction for testing the reaction of C57BL/6 splenocytes to Balb/c splenocytes. I cultured the whole splenocytes of Balb/c mice with the irradiated splenocytes of...
04 May 2015 3,524 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View