Can anybody share the protocol for the intracellular staining of mouse macrophages from brain for the anaysis of TNF-a production by Flow cytometry?

I need to analyze the TNF-a production from mouse macrophages from brain by Flow cytometry. I see there are different protocols used by people. All I see is the addition of Brefeldin A to the...

05 June 2018 1,343 6 View

What are the suitable markers for activated microglia in mice brain by Flow cytometry?

What are the suitable markers for distinguishing activated macrophages from activated microglia in mice brains by Flow cytometry?

04 May 2018 4,402 0 View

Which splenic DC subset is more specific for CD4+ T cell activation?

I want to set up the co-culture experiment using OT-II CD+T cells and splenic DC subsets for checking the CD4+ T cell activation. I was wondering out of the various splenic DC subsets, which DC...

06 July 2016 9,655 2 View

What are the best markers to differentiate between naive and activated CD8+ T cells in mice?

I want to check the activation status of CD8+ T cells from mouse spleen. I was wondering which activation markers I should use. Will CD44 and CD62 L together work to differentiate the naive CD8+ T...

06 July 2016 935 4 View

Which is the best marker to sort splenic macrophages from mice by flow cytometry?

05 June 2016 1,756 3 View

Which is the best antibody to be used for sorting splenic DCs by FACS?

I have to sort splenic DCs from mice and was wondering which antibody is best to sort splenic DCs. Can I use just mouse anti- CD11c antibody for sorting splenic DCs as CD11c+ cells. Also, how many...

03 April 2016 3,397 4 View

Is it a good idea to detect the good amount of IFN-gamma from the in vitro culture of MTB infected splenocytes?

I want to know whether it is a good idea to measure the IFN-g release by intracellular staining (Flow cytometry) from the in vitro culture of naive splenocytes infected with a specific M....

10 November 2015 9,595 9 View

Is there any other specific technique to identify microglia from mice brain other than Flow cytometry?

I usually look at microglia isolated from mice brain using the standard percol gradient method without using Collagenase/DNAse steps i.e. just meshing the brain with 10 ml syringe plunger and then...

10 November 2015 7,446 6 View

Has anybody used Zombie aqua dye (fixable viability dye for FACS) and can give me an idea about the gating of the cells?

05 June 2015 7,393 5 View

Is CFSE more sensitive method than MTT assay to detect proliferation of cells?

I am doing mixed lymphocyte reaction for testing the reaction of C57BL/6 splenocytes to Balb/c splenocytes. I cultured the whole splenocytes of Balb/c mice with the irradiated splenocytes of...

04 May 2015 3,424 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View