Do Inhibition of protein denaturation consider as a screening test for anti inflammatory activity of plants? If so why ?

Some people give an opinion that Inhibition of protein denaturation is not a relevant work and also cannot perform with plant extract. So like to get new views.

02 March 2019 1,839 9 View

Hi, which is the proper method for the inhibition of protein (albumin fraction) denaturation by plant extracts?

I am in doubt about different methods of preparations and calculations. While I performed it I got turbid material after denaturation by heating at 57 C for 3 minutes. So the absorbance at 416 nm...

08 September 2018 4,702 1 View

Can we use methanolic extract dissolved in dmso for biosynthesis of silver nano particles?

Aqueous extract is difficult to get from my plant. So I would like to try with other solvent extracts. These can dissolve only in dmso. So anybody can tell me any reaction of dmso or alcohol do...

07 August 2018 575 9 View

Is there any possibility to obtain the percentage of inhibition as above 100 after calculation?

i did my calculation for reducing power assay. Since the control value is very less than that of sample, i got % of inhibition as above 100.

02 March 2018 7,431 4 View

How to make serial dilutions or different concentrations of extracts dissolved in dmso or other solvents?

I would like to make different concenterations of my plant extract in different solvents such as 5ug, 25 ug,50 ug, 100 ug, 150ug etc per 1 ml for different assays.

01 February 2018 8,253 9 View

Is there any option to calculate total flavonoid content of my plant extracts in different solvents without using standard?

i have to know the total flavonoid content of my plant extracts in different solvents. But the standard is not available at present. So if any other options, please let me know. Thanking you in...

31 December 2017 3,264 6 View

Can anyone give the reason for getting less value for control in BSA denaturation assay?

I have done repeatedly BSA heat denaturation assay but I get more value for denatured one with sample and test than that of control. I measured in both 660 nm and 416 nm. It is not any coloured...

11 December 2017 1,970 3 View

Can anyone give an idea that whether difference in altitude and climate cause changes in plant's appearance, texture, internodal length etc.?

I have a plant which shows minute differences in different locations. This is having many species with similar properties.

10 November 2017 2,171 6 View

Can anybody give the exact absorbance (wavelength) for inhibition of protein denaturation?

I could see 660 nm and 416 nm in different papers. I have doubt that in this study whether we measure the amount of protein which is not denatured or some other content. If the absorbance gives...

10 November 2017 3,341 5 View

Which is the method for measuring absorbance, turbidity etc?

i confused whether it is colorimetry or uv spectrophotometer?

09 October 2017 5,465 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View