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Questions related from Sumin Mary Jose
Some people give an opinion that Inhibition of protein denaturation is not a relevant work and also cannot perform with plant extract. So like to get new views.
03 March 2019 1,897 9 View
I am in doubt about different methods of preparations and calculations. While I performed it I got turbid material after denaturation by heating at 57 C for 3 minutes. So the absorbance at 416 nm...
09 September 2018 4,847 1 View
Aqueous extract is difficult to get from my plant. So I would like to try with other solvent extracts. These can dissolve only in dmso. So anybody can tell me any reaction of dmso or alcohol do...
08 August 2018 664 9 View
i did my calculation for reducing power assay. Since the control value is very less than that of sample, i got % of inhibition as above 100.
03 March 2018 7,519 4 View
I would like to make different concenterations of my plant extract in different solvents such as 5ug, 25 ug,50 ug, 100 ug, 150ug etc per 1 ml for different assays.
02 February 2018 8,314 9 View
What has to use as blank for DPPH assay, nitric oxide scavenging assay, reducing power assay etc. Or what should be use as blank for antioxidant, anti inflammatory, antibacterial assays commonly...
02 February 2018 8,264 3 View
i have to know the total flavonoid content of my plant extracts in different solvents. But the standard is not available at present. So if any other options, please let me know. Thanking you in...
01 January 2018 3,334 6 View
I have done repeatedly BSA heat denaturation assay but I get more value for denatured one with sample and test than that of control. I measured in both 660 nm and 416 nm. It is not any coloured...
12 December 2017 2,043 3 View
I have a plant which shows minute differences in different locations. This is having many species with similar properties.
11 November 2017 2,236 6 View
I could see 660 nm and 416 nm in different papers. I have doubt that in this study whether we measure the amount of protein which is not denatured or some other content. If the absorbance gives...
11 November 2017 3,419 5 View
my value of sample is greater than that of standard and when I consider the formulas such as (1) Percentage inhibition = (Abs Control – Abs Sample) x 100 / Abs control (2) % Inhibition=100 −...
10 October 2017 5,270 5 View
i confused whether it is colorimetry or uv spectrophotometer?
10 October 2017 5,539 3 View
I am performing in vitro anti inflammatory works. From different articles, I found out procedures which are having variations relate to reagents in each paper.
09 September 2017 7,052 2 View
Plant is having abundant amount of mucilage. So the extract is resemble to tar. It is difficult to dissolve it for the chemical reactions using different concentrations.I tried with the same...
08 August 2017 1,460 9 View
Do Inhibition of protein denaturation consider as a screening test for anti inflammatory activity of plants? If so why ?
01 January 1970 2,501 3 View
I have seen in papers both of them together...So I am doubted whether there is any difference between both...
01 January 1970 6,441 3 View
Aqueous extract is difficult to get from my plant. So I would like to try with other solvent extracts. These can dissolve only in dmso. So anybody can tell me any reaction of dmso will affect the...
01 January 1970 5,463 5 View
