i have to know the total flavonoid content of my plant extracts in different solvents. But the standard is not available at present. So if any other options, please let me know.
You can take the value of standard from a lab fellow who might have run the standard in the past or look for the value of standard in published literature and use that.
We don't reuse the same curve of any standard, only if you work in the same conditions in your lab (the same spectrophotometer, etc). Try for example to take different curves from papers and compare , you'll see the difference. In your case, you can take the standard curve from your lab member or someone who done it before and used the same apparatus than you for your flavonoids.