i did my calculation for reducing power assay. Since the control value is very less than that of sample, i got % of inhibition as above 100.
Hi Sumin,
As you said, the control value is less than your sample, which means that your sample has a greater inhibition power. Therefore, you got a value above 100%.
I mean negative control which gives less value than that of sample not the positive control. Normally, I saw the calculation as [abs of control - abs of sample/abs of control]* 100..Is there any other possible calculation when gets less control value.
this is the most significant formula , to calculate percentage of inhibition.....if u r performing dpph assay,then u can also check your plant extract capacity to scavenge radicals by color reaction .
And I think ...percentage of inhibition should not be more than 100%...
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