This cell line were culture in L-15 medium with 20% FBS, and subculture in 1:3 ratio.
However, this cells growth very very slow!
Whether MDA-MB-361 breast cancer cell line growth slowly?
As shown in the relevant Cellosaurus entry:
http://web.expasy.org/cellosaurus/CVCL_0620
They have a doubling time of 4.3 days, so quite slow
Best
Amos
Hi. Try a different media like DMEM. Also, i use 1% antibiotic in my media with 10%FBS.
If you are using L-15 media, make sure you incubate at 0% co2.
Does the medium have L glutamine?
Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...
01 March 2021 2,957 8 View
18 February 2021 9,188 3 View
There are two plates with horizontal center crack, one with crack length of a with the other with a+da. The bottoms are fixed while the tops are under the same displacement load. For intuition,...
11 February 2021 9,543 2 View
Hello Dear Researchers, I am confused about the Difference between Second-harmonic generation and Two-photon absorption? Can anyone describe the two phenomena briefly in the physical picture? What...
24 January 2021 9,912 5 View
I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).
14 January 2021 3,805 2 View
I just developed a new theory on electromagnetic and plan to publish in a Journal. The findings are purely through strict mathematical derivation without any disputable assumptions and also...
13 January 2021 1,558 5 View
Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...
13 January 2021 8,336 4 View
I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...
31 December 2020 8,838 3 View
Hi, I use YASARA (version 17.8.15) on windows 10 and recently I am trying to install FoldX plugin on YASARA. Here is what I did: 1. Download YASARA plugin for windows (and the executable and...
24 December 2020 536 3 View
I am learning the knowledge about barge pier collision these days, and have a problem that the impact force of barge pier collision suddenly increase to the peak value in ls-dyna. But the...
05 December 2020 9,978 3 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
02 March 2021 3,060 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View