Dear/Madam
I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash the breast cancer sample before I put in liquid nitrogen in the Laboratory? .If 1X PBS or 10X PBS is not good for that? which buffer solution Is the best to wash the cancer sample before putting in liquid nitrogen?.
Washing is usually done with 1X PBS.
But, what is your next step after freezing in liquid nitrogen?
Do you want to freeze then do protein or RNA extraction?
Are you going to do any histological analysis after freezing?
The answer will differ depending on your next step.
Dear Abdullah
Thanks for helping, I am going to extract total RNA, Protein and microRNAs. I am going to look the expression of some MicroRNAs and mRNAs. Normally, the operation staff first put the breast cancer tissue in formalin. Because it is difficult to get fresh breast cancer tissue and can not be given , histologists give me a tumor in histopathological room after 5hr. my question is here, I will wash the formaline-fixed breast tissue by 1x PBS and then stay it for 10 hrs to clean from formalin and then put it in liquid nitrogen and then store in - 80. is it ok to do these processes? can the formalin fixed tissue be washed whether by 1X PBS or 10X PBS? which one is better. After washing, I put in liquid Nitrogen to dry it and then store at -80 c.
1X PBS is used usually.
However, the formalin fixation will decrease the efficiency of RNA extraction.
You should use an RNA extraction kit compatible with FFPE samples.
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