How are ways to communicate with students in the classroom to find different ways of communicating in e-learning?

18 October 2020 4,749 3 View

Does anybody has the protocol of MicroRNA purification from FFPE block tissue?

Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.

16 January 2020 9,143 2 View

How to make boxplot in R or anyother app?

Hi Any help is appreciated. How to produce a boxplot graph as in the attached image (from Gregory et al., 2019)? using R or any other software?

15 December 2019 2,208 4 View

Can anyone help me find an Instrument to measure rogers five attributes of innovation?

I am currently researching voluntary reporting and its determinants through the (DIO) theory of innovation diffusion. I need a measurement tool other than interviews such as questionnaires if...

02 December 2019 6,128 4 View

Who can help me.I need papers on the Fuzzy Activity Based costing system ?

Fuzzy cost systems generally aim to counter uncertainty in estimating cost vectors as inputs to costing systems, making cost estimates accurate, as well as increasing reliability in results. These...

08 November 2019 9,679 1 View

What do you think about distance study? Do you expect it to succeed in developing countries under the circumstances it is going through?

10 October 2019 8,101 14 View

Whether 1XPBS or 10X PBS is better for washing the breast cancer sample that placed in formalin? how many times can I wash the sample with the PBS?

Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...

03 July 2019 9,461 3 View

A tool for creating synthetic time series based on the existing one?

Hello, I have a historical time series of 72-year monthly inflows. I need to generate, say 100, synthetic scenarios using the historical data. Simple resampling (by reordering annual blocks of...

26 April 2018 4,512 6 View

Can anyone tell me how to use FASTA sequence protein to visualise its structure?

Visualising protein structure

07 October 2013 2,339 14 View

Any suggestion for PHD proposal in health sector

Good morning I hope you all doing great and safe I got Bachelor degree on health administration and master on health administration concentration in health informatics . I want to start a PHD...

01 January 1970 866 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View