Sevan Majed

6 Questions 4 Answers 0 Followers

Questions related from Sevan Majed

Does any one has any molecular biological software for designing the schematic of miRNAS like image that I provide?

Hi I want to design miRNA targets and schematic of miRNA targets for microRNA. I need a great software. Does anyone has it? if you have any software, please could you give me? Best Regards Sevan

04 April 2020 9,106 2 View

Can I synthesize single strand cDNA for miRNA from total RNA? or Can I use Total RNA extracted to isolate microRNA?

Dear/ Madam I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I...

19 January 2020 1,652 6 View

Can I use DNase I during washing microRNA Enrichment Column?

Dear/Madam I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had...

18 January 2020 7,556 3 View

Does anybody has the protocol of MicroRNA purification from FFPE block tissue?

Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.

17 January 2020 9,209 2 View

Whether 1XPBS or 10X PBS is better for washing the breast cancer sample that placed in formalin? how many times can I wash the sample with the PBS?

Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...

04 July 2019 9,547 3 View

How could find the perfect target for miRNAs?

Dear Sir/Madam I know there are some websites giving you the target predicted for microRNA. I am a little confused about them. Can anyone has a reliable websites that is helpful to get the best...

01 January 1970 1,472 3 View