Where can one find solid global data on the condition of existing concrete structures?

Dear colleagues, I am looking for literature covering the condition of concrete engineering structures in Europe and globally. I am not looking for guidelines for the condition assessment, but...

03 December 2020 6,076 2 View

Do you know any pyrolysis technology with an output up to 20.000 t coal?

The upscaling of Pyrolysis with the input material wood chips from forests could be an opportunity to produce coal for industry' process heat demand. Backround are the CO2 prices and the...

10 February 2020 7,352 8 View

Can anyone recommend a recent review of the research on effectiveness of special education as practiced in the public schools in the US ?

I am interested in studies of outcomes from special education as it is implemented normally in public schools. A great deal of data was collected under the NCLB Act but I have not seen...

15 November 2018 6,279 2 View

What are the best markers to analyze proportions of eosinophils and neutrophils by flow cytometry?

I am new working with Flow citometry and I need to analize the proportions of eosinophils and neutrophils present in the mouse airways with 2 different treatments. My sample will be a wash with...

09 February 2016 6,803 5 View

Which general primers would you recommend in order to screen gut content of spiders for invertebrate prey via next generation sequencing?

27 July 2014 5,120 6 View

Design of Experiments with R?

Hello everyone, is here someone who experience with Design of Experiments eg. statistical experiment planning in R. Is there a recomendable packadge and are there some examples how to structure...

01 January 1970 6,438 8 View

Do you care about robot cybersecurity?

Dear fellow roboticist, Many now agree that robotics is set to be the next major technological revolution. A robot can be defined as a cyber-physical system that can automate traditional and...

01 January 1970 8,326 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View