Where can I get LN-308 cell line?

Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...

20 October 2020 3,004 3 View

How can I quantify bacteria viable cells in a non-sterilized organic compost?

I'm working with the enrichment of an organic compost with PGPR, but this compost is not sterilized. How could I quantify viable cells of Azospirillum brasilense, Bacillus subtilis, and...

01 April 2020 9,486 4 View

How to model near-stall compressor operation with RANS CFD?

Hello everyone. I am looking into near-stall behaviour of the multistage axial compressor using CFD with RANS method. What is the best practice to establish if the compressor is close to stall,...

09 March 2020 8,847 8 View

What iron species are precipitating after autoclaving ferric chloride water solution?

I made 10mM FeCl3 solution in MQ water. After autoclaving it (standard 15min procedure) solution turns orange-brown. Filtering using 0.22um filter does not remove the color (but some of it is...

24 September 2019 2,407 7 View

What is a typical RFU value for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer?

Hi, quick question, What typical RFU value should I expect for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer? I made two standards (100 uL dye buffer, 98 uL...

10 April 2018 5,090 3 View

RNAprotect reagent formulation?

Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...

04 March 2018 5,089 14 View

How much does ammonium sulfate precipitation affect nucleic acid integrity?

Hello, In my current project I am at present trying to tackle an issue of rapidly stabilizing bacterial RNA in a large environmental sample. Most known stabilization reagents are not applicable in...

04 March 2018 1,589 3 View

SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data and Light Pollution Assessment

We are working with SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data, which has units of W cm-2 sr-1. The case study area is a bird sanctuary and an inhabited territory that is also used for...

14 May 2017 7,715 2 View

Is there a relation between Hadamard product and standard matrix product?

05 July 2015 5,366 7 View

Can we get the sufficient number of intensity measurements for a complex vector reconstruction in quantum tomography?

In a paper arXiv:1312.0158 the authors proved that any complex vector (thus also the quantum state vector) can be uniquely determined (up to a global phase factor) by 4N-4 generic intensity...

22 June 2015 7,125 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View