15 Questions 16 Answers 0 Followers
Questions related from Artur Zaduryan
Hey everyone, my question is maybe strange at first glance, but simple: is the rapid 16S kit's only real advantage the significantly larger 16S data amount generation? Shouldn't I be perfectly...
13 March 2024 3,860 2 View
Hi! I am prepping a sulfate reducer (Desulfovibrio sp.) medium (M63 from DSMZ https://mediadive.dsmz.de/medium/63) and I am trying to make sense of the formulation. So far my understanding...
13 February 2024 556 4 View
Hi! I am trying to understand whether it is important for me to use an RNA Cleanup kit (such as NEB Monarch #T2030S). I am isolating microbial RNA from an environmental liquid sample, and I...
11 January 2023 1,445 4 View
Hi everyone! I would like you to show off your most successful strategies to acquire bacterial / archaeal high quality intact RNA from environmental samples / mixed community (liquid...
20 October 2022 1,656 0 View
Hi, I am currently dealing with extremely low amounts of environmental microbial RNA (typically
08 February 2022 5,037 9 View
Hi, quick question, What typical RFU value should I expect for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer? I made two standards (100 uL dye buffer, 98 uL...
11 April 2018 5,264 3 View
Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...
05 March 2018 5,236 14 View
Hello, In my current project I am at present trying to tackle an issue of rapidly stabilizing bacterial RNA in a large environmental sample. Most known stabilization reagents are not applicable in...
05 March 2018 1,745 3 View
Hello! I am looking for a specific protein family homologues in different bacterial species, and I was wondering, which online free tool you are using when a similar need arises for you?...
27 January 2018 4,808 5 View
Hello! we're performing a quorum sensing secretome characterization of a couple of bacterial strains, and we have met a few issues, which we cannot explain. First, we had our Agrobacterium...
25 January 2018 145 3 View
Hello! I am preparing to conduct a quorum sensing signal molecule assay on a bacterial species, where I would like to characterize the secretome of n-acyl-homoserine lactones (AHLs) that this...
12 December 2017 5,556 2 View
Hello! Me and my partner are trying to figure out the initial mechanism of Mitsunobu azidation with PPh3/DIAD/DPPA. In any mechanism, as well as the original paper where azidation with DPPA is...
10 December 2017 9,489 3 View
Hello! I am just starting to explore self-labeling tags technologies in the course of my internship in MPIMF Heidelberg, and am playing around with the machines that are available. One is Tecan...
11 July 2017 407 3 View
Hello! I'm desperately trying to find clues to the molecular mechanism of prion replication: how do misfolded (scrapie form) PrPs actually catalyze misfolding of the normal cellular PrPs on...
19 May 2017 321 3 View
Hello! In my ongoing project I am trying to isolate the RNA for transcriptomic analysis from a giant sulfur bacterium, Achromatium oxaliferum (kindly see the project description for more details)....
01 January 1970 980 5 View
