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Questions related from Artur Zaduryan
Hi! I am trying to understand whether it is important for me to use an RNA Cleanup kit (such as NEB Monarch #T2030S). I am isolating microbial RNA from an environmental liquid sample, and I...
11 January 2023 1,436 4 View
Hi everyone! I would like you to show off your most successful strategies to acquire bacterial / archaeal high quality intact RNA from environmental samples / mixed community (liquid...
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Hi, I am currently dealing with extremely low amounts of environmental microbial RNA (typically
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Hi, quick question, What typical RFU value should I expect for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer? I made two standards (100 uL dye buffer, 98 uL...
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Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...
05 March 2018 5,214 14 View
Hello, In my current project I am at present trying to tackle an issue of rapidly stabilizing bacterial RNA in a large environmental sample. Most known stabilization reagents are not applicable in...
05 March 2018 1,720 3 View
Hello! I am just starting to explore self-labeling tags technologies in the course of my internship in MPIMF Heidelberg, and am playing around with the machines that are available. One is Tecan...
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Hello! In my ongoing project I am trying to isolate the RNA for transcriptomic analysis from a giant sulfur bacterium, Achromatium oxaliferum (kindly see the project description for more details)....
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