If the plant extract is dissolved in ethanol only means how can i administer the plant extract to the albino rats?

crude plant extract is dissolved in ethanol. But ethanol is not consumable. Is there any other way to administer the drugs to the rats.

04 May 2018 9,038 11 View

How do i prepare the plant sample for NMR analysis?

I have a crude extract of the plant powder. I need to do NMR analysis. How can i prepare the pure sample for NMR analysis from crude extract?

03 April 2018 1,956 8 View

What are the chemical solution to dissolve the crude extract?

I used benzene as a solvent to extract the plant powder? I used water, 100% DMSO, 5 and 10% Tween 80, 5 and 10% Tween 20, ethanol, methanol. But the extract does not dissolved. other than these...

03 April 2018 9,325 3 View

Can i use 5% tween 80 to dissolve plant extract (benzene used as solvent)?

Benzene extract of the plant does not dissolve in water.

03 April 2018 1,057 6 View

Is it necessary to test the toxic effect of the biosynthesized nanoparticles, even after the plant extract has been proven that has no toxic effect?

To test the toxic effect of of the silver nanoparticle synthesized using plant extract. But the crude extract of the plant had no toxic effect.

02 March 2018 9,019 9 View

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I am not aware about the UV lamp model to induce psoriasis in the laboratory rats. Is this lamp is OK to induce psoriasis?

07 August 2017 7,720 3 View

How do i identify the nature of the compound?

I had the compounds list of the plant extract, obtained through the GC-MS analysis. How do i identify whether the compounds are new or already identified compounds?

05 June 2015 2,614 0 View

What are the cell lines (which is best) to study antidiabetic activity?

I want to study the antidiabetic activity in vitro. What are the cell lines other than pancreas are the best to study the antidiabetic activity? And in the pancreas which cell line is best to give...

03 April 2015 5,221 9 View

It is possible to extract the fractions in the plant crude extract through HPLC analysis without going GCMS?

03 April 2015 3,111 4 View

Please inform me the publications about the tree ferns?

I want to know about the details of tree ferns Particularly Cyatheaceae family.

02 March 2015 632 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View