Mohamed Antar Aziz Mohamed

5 Questions 16 Answers 0 Followers

Questions related from Mohamed Antar Aziz Mohamed

What to do if I have some cloudy suspensions (aggregates) during protein concentration step just before GF step?

During protein concentration step by using Amicon tubes with 10KDa cutoff (from Merck company), I could see some cloudy suspension which indicate for protein aggregates formation. Afterwards in...

09 September 2016 3,457 12 View

EMSA technique from one week ago; my bands are perfect but free oligonucleotides did not appear.

As I mentioned my bands are sharp and perfect but no free oligonucleotides and I repeated it again using a large volume of labelled probe but I got the same result no free oligonucleotides. Why?

03 March 2013 5,356 7 View

In western blot, I use b-actin as control but sometimes I get two bands of b-actin instead of one band, why?

I use HaCat cell line

01 January 2013 7,940 21 View

After transferring on to a membrane step in western blot, my marker and protein bands are smeared. Why does this happen?

My collegues using the same protocol and same buffers are getting good results.

01 January 2013 9,716 43 View

I did RT PCR for mmp-1,procollagen and GAPDH but when I used primers for mmp-1, procollagen and GAPDH all bands are the same.

Why are all the bands the same although I used different primers for different targets?

08 August 2012 3,787 7 View