I want to study the antidiabetic activity in vitro. What are the cell lines other than pancreas are the best to study the antidiabetic activity? And in the pancreas which cell line is best to give the accurate result.
That's depends on what are you looking for. Insulin signalling can be assessed using muscle (e.g. C2C12 cells) or liver (e.g. HepG2) cell lines. You should also consider primary cells (more physiological relevant). Good luck!
It depends on what is your aim. we have used insulin responsive cell lines like MIN6, RIN5f to study effect on insulin secretion. we have used differentiated L6 myocytes and NIHT3T cell lines to study insulin sensitivity and insulin signalling.
yes, it depends on your hypothesis, if you want to investigate glucose uptake so it is better to use muscle derived cell lines and in a lesser extent adipose derived cells. However, if your goal is to study reduction in glucose production as antidiabetic effect go to liver derived cell lines.
Hi Elavarasi. It depends on your goal. In general, you can use muscle cells (better for the glucose uptake since they have glut-4 which translocation is insulin-regulated) or adipocytes (similar) or liver cells.
Best regards,
FM
where are the cell lines (diabetic) available ? what is the cost of testing anti diabetic activity
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