Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View

I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

Can anybody help to find a tool for assessing study habits of students?

It is for my research purpose

21 December 2020 833 3 View

How to identify theories to write in theoretical framework chapter?

I am working on Entrepreneurship Education topic. I want to write theoretical and conceptual framework. Can you please tell me the procedure to write theoretical framework.

15 May 2020 1,101 1 View

Hello everyone. my question is about literature review. While writing literature review in thesis is it compulsory to write everything by our own?

I am writing literature review and need a suggestion regarding how much I can copy from previous study and how much I can write my own. If I m writing by own what are the important points to be...

11 May 2020 8,385 11 View

From where I can purchase Cutaneous Squamous Cell Carcinoma (SCC) cell lines, SSC12 and SSC13?

These are Cutaneous Squamous Cell Carcinoma derived cell line

11 March 2020 7,344 7 View

If a guideRNA is cloned in a given plasmid, downstream of a U6 promoter would it be able to express in bacteria, specifically E coli in this case?

Alternatively I would like to know if there's any bacterial promoters that are recommended for expressing small RNA molecules?

19 February 2020 1,101 4 View

Could you share research papers on comparing effectiveness of online learning with that of face-to-face learning?

My Masters level student has been working on comparing effectiveness of online learning with that of face-to-face learning. Can you please share papers or pieces of scholarly advice with us? Your...

19 December 2019 932 9 View

I added a paper this moring but why doesn't it show up?

The title of the paper is: arXiv and the Symbiosis of Physics Preprints and Journal Review Articles: A Model

01 April 2019 5,098 2 View

What policies may bring about sustainable reform and development?

Social disparities rise amid volatile economic and political times today. What may be constructive efforts in policy form to start resolving issues that cause such state of affairs?

16 March 2019 1,086 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View