If a video is available, it will assist me more. And the adipocyte tissue culture procedures
Please i need a proper protocol to isolate intramuscular preadipocyte from the rabbit
16 April 2024 9,395 0 View
my research is focused on IMF of rabbit. however, unlike other livestock like beef cattle and pork, it is difficult to see IMF deposition on the longissmus muscle of rabbits. please i need a...
29 January 2024 2,157 0 View
I am currently on my PhD research, which focused on validating some identified long non-coding RNAs that regulates intramuscular fat deposition in rabbit, but I lack the basic experimental skills...
17 January 2024 8,020 2 View
Is Physiotherapy intervention really needed during the acute phase of Neurosyphilis? If yes, what is the best management approach (evidence-based)?
10 September 2022 8,022 2 View
To enable us generate place records for Nigeria and Africa for early response in the fight against NCDs and Chronic disease. To show that Geographic Information matters in disease control.
13 July 2021 6,875 0 View
Hi From experience does anyone know if there is a link between cell passage number and gene-editing efficiency? I suspect there might be an inverse relationship (ie high passage number = lower...
04 July 2021 4,771 2 View
Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...
29 January 2021 1,620 2 View
I mean, a study plan that draws up a preliminary timetable for completing PhD research studies. It could be in an Excel sheet or MS-word format.
14 December 2020 1,996 3 View
I would like to know if T7 in vitro transcription can be effectively terminated by pol II terminators (specifically bGH poly (A) signal)? And in reverse can a pol II promoter (eg CMV promoter)...
08 June 2020 9,595 0 View
Alternatively I would like to know if there's any bacterial promoters that are recommended for expressing small RNA molecules?
19 February 2020 1,246 4 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View