Does anybody know companies/institutes/biobanks that distribute this cell line? Also, if anyone can suggest cell lines with aggressive/metastatic properties, that will be really helpful.
HI,
Please find the attached link for MHCC-97 Cell line
http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM501782
We had some issue with delivery and it is unexpectedly longer to arrive which was not what the company initially promised. I am using a Corning brand and I knew that I have the option of coating a...
07 August 2018 1,002 0 View
Hi everyone.. is it possible to analyse our data saved as JPEG in ImageJ? I did tumourspheres culture under hypoxic condition and have to take the pictures of my spheres in multiple 6 well plates,...
02 March 2018 3,952 4 View
It is quite known that it requires up to 24 hours exposures in hypoxic condition (1% O2) to establish acute hypoxia in cancer cells cultured in monolayer, whereas > 24 hours (2-5 days) have...
10 November 2017 6,172 2 View
I am staining for E-cadherin (membrane) and vimentin (intracellular) marker for my EMT study. Anybody has a working protocol where I can do these simultaneous staining on 1 sample. Or is it better...
10 November 2016 7,160 2 View
I am having trouble getting enough protein to run SDS-PAGE, I barely get 50-60 ug total protein as my total concentration in a final volume of 150 ul. I grow my tumoursphere in Corning ULA 6 well...
01 February 2016 6,619 3 View
I am culturing liver cancer cell line in serum free tumour sphere medium (DMEM/F12 + EGF + bFGF) and culturing them for at least 7 days to enrich for cancer stem cells. Do I need to change the...
10 November 2015 746 3 View
I am treating my cells using increasing concentrations of drug, and the drug is dissolved in 100% DMSO. I limit the concentration of the DMSO in the final media with the drug to 0.5% for the...
10 November 2015 6,152 2 View
Just wondering.
10 November 2015 7,929 3 View
I know from ATCC/JCRB depository saying that these cells are different based on their source and the presence of HBV/HCV. What kind of other consideration when I'm looking into working with more...
08 September 2015 1,033 7 View
Hi, I am trying to generate a spheroid to culture HepG2 cells , and my objective is to evaluate the effect of different culture conditions on the marker expression. Not really on the optimisation...
07 August 2015 9,769 18 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Please go through my Abstract. I can also share a proposed Thesis Outline.
04 August 2024 2,077 0 View