Can anyone here from Malaysia borrow me 6-well ultra-low attachment plate for 3D culture?

We had some issue with delivery and it is unexpectedly longer to arrive which was not what the company initially promised. I am using a Corning brand and I knew that I have the option of coating a...

07 August 2018 909 0 View

Can we analyse our JPEG data using ImageJ?

Hi everyone.. is it possible to analyse our data saved as JPEG in ImageJ? I did tumourspheres culture under hypoxic condition and have to take the pictures of my spheres in multiple 6 well plates,...

02 March 2018 3,857 4 View

How do we determine the duration for acute and chronic hypoxia exposures in tumourspheres/3D culture of cancer stem cells?

It is quite known that it requires up to 24 hours exposures in hypoxic condition (1% O2) to establish acute hypoxia in cancer cells cultured in monolayer, whereas > 24 hours (2-5 days) have...

10 November 2017 6,019 2 View

How do I perform double staining from one sample when one protein requires permeabilisation and the other does not?

10 November 2016 7,071 2 View

How do I increase protein yield when doing total protein extraction on tumourspheres?

01 February 2016 6,544 3 View

Do I need to change media for cancer stem cell tumoursphere culture everyday?

I am culturing liver cancer cell line in serum free tumour sphere medium (DMEM/F12 + EGF + bFGF) and culturing them for at least 7 days to enrich for cancer stem cells. Do I need to change the...

10 November 2015 677 3 View

Why does 3D culture promote differentiation for embryonic stem cells but enrich for stem-like cells in cancer cells?

Just wondering.

10 November 2015 7,806 3 View

Do I need to use different vehicle controls for my untreated cells?

I am treating my cells using increasing concentrations of drug, and the drug is dissolved in 100% DMSO. I limit the concentration of the DMSO in the final media with the drug to 0.5% for the...

10 November 2015 5,995 2 View

What are the difference between different HCC cell lines (HepG2, Huh7, PLC/PRF/5)?

I know from ATCC/JCRB depository saying that these cells are different based on their source and the presence of HBV/HCV. What kind of other consideration when I'm looking into working with more...

08 September 2015 887 7 View

Can anyone recommend a good quick spheroid formation protocol in 6- well plates?

Hi, I am trying to generate a spheroid to culture HepG2 cells , and my objective is to evaluate the effect of different culture conditions on the marker expression. Not really on the optimisation...

07 August 2015 9,538 18 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

02 March 2021 3,060 3 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View