Ain Zubaidah Ayob

12 Questions 4 Answers 0 Followers

Questions related from Ain Zubaidah Ayob

Can anyone here from Malaysia borrow me 6-well ultra-low attachment plate for 3D culture?

We had some issue with delivery and it is unexpectedly longer to arrive which was not what the company initially promised. I am using a Corning brand and I knew that I have the option of coating a...

08 August 2018 985 0 View

Can we analyse our JPEG data using ImageJ?

Hi everyone.. is it possible to analyse our data saved as JPEG in ImageJ? I did tumourspheres culture under hypoxic condition and have to take the pictures of my spheres in multiple 6 well plates,...

03 March 2018 3,925 4 View

How do we determine the duration for acute and chronic hypoxia exposures in tumourspheres/3D culture of cancer stem cells?

It is quite known that it requires up to 24 hours exposures in hypoxic condition (1% O2) to establish acute hypoxia in cancer cells cultured in monolayer, whereas > 24 hours (2-5 days) have...

11 November 2017 6,133 2 View

How do I perform double staining from one sample when one protein requires permeabilisation and the other does not?

I am staining for E-cadherin (membrane) and vimentin (intracellular) marker for my EMT study. Anybody has a working protocol where I can do these simultaneous staining on 1 sample. Or is it better...

11 November 2016 7,133 2 View

How do I increase protein yield when doing total protein extraction on tumourspheres?

I am having trouble getting enough protein to run SDS-PAGE, I barely get 50-60 ug total protein as my total concentration in a final volume of 150 ul. I grow my tumoursphere in Corning ULA 6 well...

02 February 2016 6,601 3 View

Where can I get metastatic cell line MHCC97?

Does anybody know companies/institutes/biobanks that distribute this cell line? Also, if anyone can suggest cell lines with aggressive/metastatic properties, that will be really helpful.

01 January 2016 8,862 1 View

Do I need to change media for cancer stem cell tumoursphere culture everyday?

I am culturing liver cancer cell line in serum free tumour sphere medium (DMEM/F12 + EGF + bFGF) and culturing them for at least 7 days to enrich for cancer stem cells. Do I need to change the...

11 November 2015 731 3 View

Do I need to use different vehicle controls for my untreated cells?

I am treating my cells using increasing concentrations of drug, and the drug is dissolved in 100% DMSO. I limit the concentration of the DMSO in the final media with the drug to 0.5% for the...

11 November 2015 6,108 2 View

Why does 3D culture promote differentiation for embryonic stem cells but enrich for stem-like cells in cancer cells?

Just wondering.

11 November 2015 7,891 3 View

What are the difference between different HCC cell lines (HepG2, Huh7, PLC/PRF/5)?

I know from ATCC/JCRB depository saying that these cells are different based on their source and the presence of HBV/HCV. What kind of other consideration when I'm looking into working with more...

09 September 2015 984 7 View

Can anyone recommend a good quick spheroid formation protocol in 6- well plates?

Hi, I am trying to generate a spheroid to culture HepG2 cells , and my objective is to evaluate the effect of different culture conditions on the marker expression. Not really on the optimisation...

08 August 2015 9,743 18 View

Can anyone recommend a good unconjugated CD133 for multiple applications?

I am planning to do western blot for a start to analyse CD133 expressions in my cells, and therefore are using unconjugated antibody, which I hoped can be used for other applications such as...

02 February 2015 5,404 2 View