When reporting mediation in a manuscript being submitted for publication, what have you found to be the easiest way to create your path diagram?

I am most familiar with using text boxes in WORD but I wasn't sure if that would be acceptable.

11 January 2021 3,585 1 View

What method is more useful for prepare metal/ metal oxide Nanocomposites?

If possible any easy preparation method

31 December 2020 1,662 3 View

Advice on the culturing of EL4 lymphoma cell line?

Hi all, this is my first-time culturing lymphoma cell line. I obtained a frozen vial of EL4 (in the background of B6 mice) lymphoma cell line, and I am the only one culturing this line in the...

04 November 2020 932 3 View

How to find a partner university for an Academic conference?

I am organizing a conference for the first time and I would like to partner with an University for the conference. What are the things that I should do to invite/request a university. Whom should...

04 October 2020 7,530 3 View

How does RNA mini kit get rid of DNA without using additional DNase treatment?

Hello everyone. I am an undergraduate student majoring in cardio-thoracic technology. I'm now working on my Bachelor's thesis that i use real time PCR (gene expression study between drug-treated...

11 August 2020 4,594 9 View

Can any one help me to perform EWAS ?

We have conducted an experiment on epigenetic allele identification and quantification for some quantitative trait. we are trying to map epigenes using epigenome association mapping (EWAS)....

10 August 2020 4,170 3 View

Application of machine learning in agriculture research?

Advances in data science throwing its impact on agriculture research data analysis. looking to understand applications of machine learning concepts in agriculture research. Thanks

29 June 2020 6,959 6 View

Behaviour, inducing stress in terrestrial invertebrates?

Hi, I found a number of behavioural papers studying anxiety behaviour following stress. Stress was induced by exposure to predators, agonistic interaction or electric shocks. But these are almost...

24 June 2020 309 1 View

How to find open access journals with no publication fees to publish a work?

How can a student be able to find open access journals with no publication fees to publish their work? A step by step procedure will be most appreciated.

04 May 2020 6,700 3 View

Is there any correlation between the impact factor of scientific journals and the average rate and time for rejection?

Is there any correlation between the impact factor of scientific journals and the average rate and time for rejection. Many high impact journals reject submissions even before peer review.

04 May 2020 262 14 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Alternatives to .45 um filter to isolate MS2 from lysed E. coli?

Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...

02 February 2021 807 3 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View