How Islamic State recruited European experts?

I am looking on literature analysing how Islamic State recruited civilian experts as computer technician, experts in managing public goods, doctors, nurses... from Western countries. Is there...

21 January 2021 6,148 10 View

Any good Foxp3 Ig for WB?

Does anybody know the best antibody for the detection of Foxp3 in Western Blot after lysis of cells expressing Foxp3? Thank you in advance.

07 September 2017 4,290 1 View

Is there is a selective medium for a clear isolation of Streptococcus thermophilus from samples full of other streptococci/enterococci?

I am looking for an agar medium suitable to S. thermophilus growth but not for other Sptreptococci. I know about: ST medium (other strep would grown nicely maybe I could change the carbon source...

06 February 2017 9,482 3 View

Recellularization protocol of colon decellularized mucosa using CRC cell line. What is the best setup?

I'm trying to recellularize 0.5 cm2 of decellularized colon mucosa with colorectal cancer cell line HT-29 and HCT-15. These cell line survive after seeding 1 day and then die. Any suggestion? Any...

24 May 2016 927 3 View

What are the critical issues that we should focus on to reach a CO2 free urban freight distribution?

The White Paper on Transport (2011) has set as an objective to reach buy 2030 an essentially CO2 free urban freight distribution in major cities. What are in your opinion the most promising...

10 November 2015 5,656 11 View

Which is the minimum number of samples required by the referee in a microarray study?

Hi everyone, In a microarray study where for example you compare the gene expression of a certain type of cancer in the population A with the population B, which is the minimum number of cases...

27 January 2015 9,454 6 View

What tools or software can be used to give meaning to a list of transcription factors?

With microarray experiments I got a list of about 100 transcription factors differentially expressed. I would like to give my list biological significance. Are there any tools or databases that...

16 March 2014 9,410 6 View

Can I use the "observed-to-expected CpG ratio" to reduce the list of DMR (differentially methylated region)?

I'm studying DNA methylation using microarrays. I got a long list of DMR and would like to reduce its size. Is it correct to exclude all DMR with an observed-to-expected CpG ratio

03 March 2014 8,807 3 View

How realistic do you think is the adoption of the "physical internet" concept to urban distribution in city centers?

Can the way physical objects are moved, stored, realized, supplied and used throughout the world be economically, environmentally and socially improved in order to guarantee sustainability via a...

20 February 2014 3,189 3 View

What are the under researched issues in Urban freight transport?

Several issues are not appropriately dealt with in urban freight transport especially due to the difficulty in gathering appropriate data but some are not appropriately studied due to a clear...

06 February 2014 5,177 17 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View