Where can I find CAPRI targets "for scorers"?

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Can anyone have suggestions to solve loss of low MW proteins in homemade polyacrylamide gels?

I know that is not a transfer problem because when I use precasted gradient gel the transfer is complete. Once I use Homemade gel I have a loss in LW molecular proteins (see attachment). During...

07 May 2024 7,590 0 View

Were IS Western foreign fighters feared by local civilians?

Dear, I am aware of literature regarding the correlation between higher level of violence against civilians and foreign fighters presence (for example Bakke 2014, Moore 2019, Doctor and Willingham...

01 June 2022 3,771 3 View

What does the importance of the "fundamental rule" (the rule of free associations) depend on?

Why should the patient indulge in free association? I am asked what are the theoretical motivations that, more than a hundred years after its birth, still characterize this fundamental assumption...

10 January 2022 8,044 3 View

ANSYS FLUENT - Air cooling of an object with an assigned starting temperature?

Hello to everyone. I recently asked about the same topic, thinking everything worked and I was just in need of the correct report; it was not correct. My goal were to make a transient simulation...

24 April 2021 9,478 6 View

ANSYS FLUENT - Cooling in perturbated air flow?

Hello to all the researchers; I'm trying to do a simulation in Ansys Fluent in order to understand what is the thermal behavior of brake discs in a complete model of a F1 car. The CFD setup is...

20 April 2021 7,719 3 View

How Islamic State recruited European experts?

I am looking on literature analysing how Islamic State recruited civilian experts as computer technician, experts in managing public goods, doctors, nurses... from Western countries. Is there...

21 January 2021 6,419 10 View

Tin oxide as gate insulator (SnO2) ?

Good Morning, I have been trying to understand if by any chances it could be possible to use Tin-Oxide as a gate insulator, from my understanding it would be required for such film to be...

02 April 2020 238 0 View

Weird b value in raw DTI data?

Dear all, After checking raw Diffusion-Tensor Imaging (DTI) data from some pilots, I noticed how systematically the first two directions show a b = 2150 in place of 2200. Has it ever happened to...

09 December 2019 8,894 4 View

Best ways to use Virtual Reality in Rehabilitation?

I'm looking for the best way to use a VR system in a motor rehabilitation protocol for post-stroke patients. Thanks to everyone who will respond!

02 December 2018 5,213 6 View

Where can I find open material about the Ethiopian gvt attempt to create an artificial language, "Wagagoda", by mixing Wolaitta, Gamo, Gofa, Dawro?

In the '90, Ethiopian government tried to create an Esperanto-style language in the Wolaitta region by mixing four different languages: Wolaitta, Gamo, Gofa, Dawro. I need more material to...

14 June 2018 915 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

How can CHARMM-GUI be used for protein-ligand interaction ?

My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.

24 June 2024 5,044 1 View

How to predict Protein structure and choose N or C terminal tagging?

Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...

24 June 2024 8,622 6 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View

Integral Membrane Protein Purification Help?

Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...

30 May 2024 7,604 3 View