How can pressure commands be applied randomly in Lamps?

Hello everyone. I wrote a program in Lammps that applies pressure on three axes separately. Since in reality the pressure is applied randomly on each of the three axes; How do I write the program...

29 December 2020 7,072 1 View

Repeated N-fold cross validation?

Hi! I am a bit confused with the term of "repeated" in repeated CV. In a typical cross validation problem, let's say 5-fold, the overall process will be repeated 5 times: at each time one subset...

25 November 2016 3,019 5 View

What is a suitable panel for normal/cancerous liver cell lines?

Hi, I plan to do some gene silencing in HepG2 cell line. However, to draw a profound comparison between normal and cancerous liver (in this case, hepatocellular carcinoma) phenotypes, a normal...

19 September 2015 2,029 5 View

Which parameters affect the choice of a tumor cell line?

There are various cell lines in literature from different cell collections (ATCC, DSMZ, etc.) or personal donations extracted from patients or just a gift from a generous researcher. However, not...

11 September 2015 6,889 6 View

How to extract gene expression and proteomics data from Human Protein Atlas?

I am trying to extract some cell-specific transcriptomic/proteomic data for cancerous tissues from Human Protein Atlas, however, it did not work out. I appreciate any cues in doing so, and if...

14 August 2015 5,233 4 View

Remove L-Arginine?

Is there any appropriate way to remove L-arginine from a buffer? I need to measure protein concentration in a buffer containing L-arginine. As L-arginine interacts with bradford buffer (or in 280...

27 May 2013 670 10 View

How can we concentrate small volumes of a protein solution?

Is there any special centrifuge filter or another instrument for the purpose of tiny volumes?

04 May 2013 8,051 15 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View