Where can I buy coformycin from?

More Lakshmeesha Kempaiah Nagappa's questions See All

How to calculate thermal conductivity using RVE analysis?

Dear all, How to calculate thermal conductivities of composites using 3DFEA based RVE analysis, can anyone suggest the procedure? in Abaqus CAE Best regards Nagappa

13 April 2021 555 4 View

How to get good clear slide mount of eriophyid mite collected from a medicinal plant?

Recently I have collected eriophyid mites from leaf erineum of a medicinal plant. When I prepare slide in Hoyer's medium, it is not getting cleared fully, instead I could see fine oil globblets on...

26 September 2020 9,627 5 View

How to calculate the volume of each/set of elements in abaqus?

Dear abaqus user i have created 3d Model and assigned material properties. so that i want to calculate volume of some selected elements may be individual/set of elements. can anyone explain...

05 September 2019 9,583 3 View

Is there growth difference between ade1 and ade2 knockout yeast?

Hi, I am interested in nucleotide metabolism and performed serial dilution and spotting using ade1 and ade2 knockout yeast strains. it seems like ade1 grows slower than ade2 eventhough they are...

19 February 2018 5,302 2 View

Query on tet off promoter in yeast?

I want to use a tet off promoter system in S. cerevisiae using a centromeric plasmid. What is the concentration of tetracycline I can use to keep the promoter Off? Doxycycline can be used upto 40...

08 February 2018 8,392 0 View

"yeast secretory expression of heterologus proteins"?

Hi, I am trying to secrete a heterologous protein in S. cerevisiae using alpha mating factor signal peptide at the N-terminus and GFP at c-terminus. The plasmid is a 2 micron (Ura selection)...

17 September 2017 2,801 6 View

Generation of sequence alignment and Phylogenetic tree (for protein)

I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...

01 June 2017 7,121 3 View

What is the probability of getting revertants after yeast transformation?

Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...

05 February 2016 460 2 View

Can promoter or terminator be swapped between different yeasts?

I am trying to make a plasmid having pichia GAPDH promoter and AOX terminator and use it in saccharomyces cereviseae? has anyone come across an article where this kind of attempt has been made. I...

16 December 2015 8,385 2 View

How can I prevent aberrant RNA splicing in a yeast heterologous system?

I am trying to express a plasmodium protein in yeast whose gene is 2 kb but when I do RT PCR I get a 450 bp band. This was sequenced and I found that it has initial 430 bp followed by final 20 bp...

29 May 2014 7,121 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Can you recommend provider Intermediate laboratory test for hydrocarbon, polyethylene & polypropylene?

Accredited laboratory of JV "Uz-Kor Gas Chemical" LLC (Uzbekistan) is looking for providers to conduct interlaboratory comparison tests on gas chromatographic analysis of hydrocarbons (component...

25 July 2024 8,108 0 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

16 July 2024 6,964 0 View