How to get good clear slide mount of eriophyid mite collected from a medicinal plant?

Recently I have collected eriophyid mites from leaf erineum of a medicinal plant. When I prepare slide in Hoyer's medium, it is not getting cleared fully, instead I could see fine oil globblets on...

26 September 2020 9,322 5 View

"yeast secretory expression of heterologus proteins"?

17 September 2017 2,663 6 View

Generation of sequence alignment and Phylogenetic tree (for protein)

I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...

01 June 2017 6,942 3 View

What is the probability of getting revertants after yeast transformation?

Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...

05 February 2016 349 2 View

Can promoter or terminator be swapped between different yeasts?

I am trying to make a plasmid having pichia GAPDH promoter and AOX terminator and use it in saccharomyces cereviseae? has anyone come across an article where this kind of attempt has been made. I...

16 December 2015 8,246 2 View

How can I prevent aberrant RNA splicing in a yeast heterologous system?

I am trying to express a plasmodium protein in yeast whose gene is 2 kb but when I do RT PCR I get a 450 bp band. This was sequenced and I found that it has initial 430 bp followed by final 20 bp...

29 May 2014 7,000 3 View

How can we implement monte carlo simulation in abaqus easyPBC ?

Dear Sadik Suppose I need to run 20 random simulations by varying the input fiber properties such as youngs modulus E11=150 +/-2 (20 random E11 value within a given range) other elastic...

01 January 1970 2,841 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

Is it possible to do optimization for a submodel in Abaqus?

I'm trying to run an optimization in Abaqus for a submodel with no luck, jobs are not being create for submission. Is this even possible? or is Abaqus limited to do Opt. for the model itself...

24 February 2021 4,908 5 View