How to get good clear slide mount of eriophyid mite collected from a medicinal plant?

Recently I have collected eriophyid mites from leaf erineum of a medicinal plant. When I prepare slide in Hoyer's medium, it is not getting cleared fully, instead I could see fine oil globblets on...

26 September 2020 9,322 5 View

How to calculate the volume of each/set of elements in abaqus?

Dear abaqus user i have created 3d Model and assigned material properties. so that i want to calculate volume of some selected elements may be individual/set of elements. can anyone explain...

05 September 2019 9,410 3 View

"yeast secretory expression of heterologus proteins"?

17 September 2017 2,663 6 View

Generation of sequence alignment and Phylogenetic tree (for protein)

I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...

01 June 2017 6,942 3 View

What is the probability of getting revertants after yeast transformation?

Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...

05 February 2016 349 2 View

Can promoter or terminator be swapped between different yeasts?

I am trying to make a plasmid having pichia GAPDH promoter and AOX terminator and use it in saccharomyces cereviseae? has anyone come across an article where this kind of attempt has been made. I...

16 December 2015 8,246 2 View

How can I prevent aberrant RNA splicing in a yeast heterologous system?

I am trying to express a plasmodium protein in yeast whose gene is 2 kb but when I do RT PCR I get a 450 bp band. This was sequenced and I found that it has initial 430 bp followed by final 20 bp...

29 May 2014 7,000 3 View

How can we implement monte carlo simulation in abaqus easyPBC ?

Dear Sadik Suppose I need to run 20 random simulations by varying the input fiber properties such as youngs modulus E11=150 +/-2 (20 random E11 value within a given range) other elastic...

01 January 1970 2,841 3 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

Is MOPS buffer needed for in vitro horizontal gene transfer in thr coculture of two different pathogenic bacterial species?

Actually i am working on possibilities of horizontal gene transfer of resistant gene in broth coculture of two different types of bacteria, and the protocol i follow involves the use of MOPS...

19 January 2021 4,566 2 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

What are the insilico tools available to recognize transcription binding sites on a promoter ?

I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...

11 January 2021 7,992 3 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View

How can I insert antibody sequences into yeast cell to achieve stable and large quantity production?

I have the sequence of the light chain and heavy chain of a certain antibody. I would like to know how to express this antibody in yeast cells to achieve stable and large quantity production. It...

04 January 2021 9,669 2 View