How to get good clear slide mount of eriophyid mite collected from a medicinal plant?

Recently I have collected eriophyid mites from leaf erineum of a medicinal plant. When I prepare slide in Hoyer's medium, it is not getting cleared fully, instead I could see fine oil globblets on...

26 September 2020 9,322 5 View

How to calculate the volume of each/set of elements in abaqus?

Dear abaqus user i have created 3d Model and assigned material properties. so that i want to calculate volume of some selected elements may be individual/set of elements. can anyone explain...

05 September 2019 9,410 3 View

"yeast secretory expression of heterologus proteins"?

17 September 2017 2,663 6 View

Generation of sequence alignment and Phylogenetic tree (for protein)

I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...

01 June 2017 6,942 3 View

What is the probability of getting revertants after yeast transformation?

Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...

05 February 2016 349 2 View

How can I prevent aberrant RNA splicing in a yeast heterologous system?

I am trying to express a plasmodium protein in yeast whose gene is 2 kb but when I do RT PCR I get a 450 bp band. This was sequenced and I found that it has initial 430 bp followed by final 20 bp...

29 May 2014 7,000 3 View

How can we implement monte carlo simulation in abaqus easyPBC ?

Dear Sadik Suppose I need to run 20 random simulations by varying the input fiber properties such as youngs modulus E11=150 +/-2 (20 random E11 value within a given range) other elastic...

01 January 1970 2,841 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View