11 Questions 12 Answers 0 Followers
Questions related from Lakshmeesha Kempaiah Nagappa
Hi, I am interested in nucleotide metabolism and performed serial dilution and spotting using ade1 and ade2 knockout yeast strains. it seems like ade1 grows slower than ade2 eventhough they are...
20 February 2018 5,336 2 View
I want to use a tet off promoter system in S. cerevisiae using a centromeric plasmid. What is the concentration of tetracycline I can use to keep the promoter Off? Doxycycline can be used upto 40...
09 February 2018 8,420 0 View
Hi, I am trying to secrete a heterologous protein in S. cerevisiae using alpha mating factor signal peptide at the N-terminus and GFP at c-terminus. The plasmid is a 2 micron (Ura selection)...
18 September 2017 2,834 6 View
I did a blast search with a sequence of my protein of interest. I got around 4000 sequences in the result. How do I eliminate redundant sequences and multiple entries for the same organism from a...
02 June 2017 7,147 3 View
I require a reagent called coformycin. I am not finding it in sigma or cal biochem. Is there any other company available? This is an inhibitor of adenosine deaminase.
21 December 2016 5,555 2 View
I have planned an experiment where I intend to increase circulating levels of adenosine / s-adenosyl methionine in the mouse blood stream and check its effect on plasmodium berghei? Should I put...
09 March 2016 591 4 View
Hi, I am working with a yeast strain which is knockout for a gene of interest (AMP Deaminase) (gene replaced by g418 casette). this strain cannot grow on S-adenosyl methionine (SAM). I transformed...
06 February 2016 480 2 View
I am trying to make a plasmid having pichia GAPDH promoter and AOX terminator and use it in saccharomyces cereviseae? has anyone come across an article where this kind of attempt has been made. I...
17 December 2015 8,413 2 View
I have a haploid yeast strain having a gene of interest replaced by G418 marker conferring resistance for geneticin. I need to remove this marker and replace with another gene (neither an...
07 December 2015 6,958 4 View
I am trying to express a plasmodium enzyme (83kda) in pichia pastoris x33 strain using pGAPZ alpha A vector. I don't see any protein secreted in the media nor in the cell fraction (western blot...
15 July 2014 7,070 1 View
I am trying to express a plasmodium protein in yeast whose gene is 2 kb but when I do RT PCR I get a 450 bp band. This was sequenced and I found that it has initial 430 bp followed by final 20 bp...
30 May 2014 7,143 3 View
