I've been using the autotracing algorithm APP2 within Vaa3D to segment neurites in a three-dimensional image dataset. The traced neurites that come out of this segmentation possess seemingly random coloration. I was wondering if anyone has experience with APP2 and could tell me what the colors mean. Are they supposed to denote separate trees? If so, why are many of my neurites blue (even when seemingly not connected)? For clarity, I have attached a screenshot from one of my segmentation efforts. I'd very much appreciate any help you can provide!

More Logan Thrasher Collins's questions See All
Similar questions and discussions