Logan Thrasher Collins

10 Questions 2 Answers 0 Followers

Questions related from Logan Thrasher Collins

Is glycol methacrylate plastic translucent to x-rays between 15 and 30 keV?

I want to embed a 2 mm thick tissue sample (with a polymerized diaminobenzidine stain) into glycol methacrylate and use synchrotron phase contrast x-ray microtomography to image the tissue....

17 January 2024 9,539 5 View

What cell lines does AAV9 transduce efficiently other than CHO-Lec2?

I'm looking for some cell lines that AAV9 will transduce efficiently at 10^6 MOI besides CHO-Lec2. I've already tested CHO-Lec2 and found decent transduction levels (~20%). But now, I would like...

20 November 2023 9,631 1 View

How do I remove AAVs from mixture of adenovirus and AAV using size-based method?

I have a mixture of adenovirus and AAV. I would like to remove the AAVs and leave only the adenovirus. Due to unique circumstances (that are unfortunately proprietary), I am unable to use affinity...

04 October 2022 6,568 2 View

How do I better visualize protein interactions at edges of periodic box in VMD?

I have a 500 ns molecular dynamics trajectory with several proteins in a membrane. The entire system is enclosed in a periodic box. Unfortunately, my proteins are only interacting around the...

12 November 2020 7,243 3 View

How expensive are commercially available customized molecular dynamics services?

I am interested in potentially using commercially available customized HPC molecular dynamics simulations. However, I cannot find pricing information on the websites of the vendors. Could someone...

08 June 2020 9,423 3 View

Does EDC/NHS coupling act on primary amides or only primary amines?

I want to use a coupling reagent (possibly EDC/NHS) to link gold nanoparticles to primary amines and NOT primary amides. There are both primary amines and primary amides in my sample, but I want...

17 January 2020 7,594 4 View

When using Vaa3D's APP2 plugin, what does the coloration of traced neurites mean?

I've been using the autotracing algorithm APP2 within Vaa3D to segment neurites in a three-dimensional image dataset. The traced neurites that come out of this segmentation possess seemingly...

23 June 2019 9,522 0 View

Where can I find antibodies against cell-surface biomarkers in honeybee neurons?

I'd like to tag honeybee neurons with antibodies for imaging purposes, so I'm looking for a commercial provider of antibodies which are reactive with cell-surface biomarkers found in the honeybee...

17 October 2018 2,528 0 View

What freeware would you recommend for tracing neurons in a tiff stack?

I've acquired a stack of tiff images from a volume of brain tissue. I'm trying to find an automated program (not semiautomated) which will allow me to trace the neurites within this volume.

20 September 2018 1,464 1 View

What is the "pore size" of brain tissue?

I was wondering about the ECM "pore size" within rat brains (in the context of nanoparticle diffusion). In addition, I would like to know if the fixation/treatment method impacts the tissue's...

16 June 2018 6,498 9 View