How to preserve best PBMC samples for performing FACS staining later ON?

31 December 2017 7,664 8 View

How to remove fat from blood to get clean plasma for cfDNA analysis?

Blood from some donors are fatty, after centrifugation an upper layer forms which is difficult to remove, in order to get clean plasma for cfDNA analysis. Any advice? Thanks!

01 February 2017 5,796 5 View

Can you please recommend me any free software for pie charts?

Any free software for pie charts for quality images, thank you!

11 December 2016 2,572 10 View

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I am wondering whether I can modify any step to increase yield, thanks a lot!!!

09 October 2016 2,579 0 View

Christmas gift!!! Can you recommend me any free software for pie charts?

Any free software for pie charts for quality images, thank you!

10 November 2015 256 1 View

How can I improve ethanol removal when precipiating DNA?

I want to remove most of ethanol following DNA precipitation, thanks!!!

06 July 2015 4,322 6 View

How can I improve DNA solubilization when using DNAzol?

How can I improve ethanol removal from final suspension?

06 July 2015 8,128 2 View

Can anyone recommend me an easy software for drawing?

I am preparing some figures that require more elaborate schemes & drawings

05 June 2014 4,780 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View