How to preserve best PBMC samples for performing FACS staining later ON?

31 December 2017 7,664 8 View

How to remove fat from blood to get clean plasma for cfDNA analysis?

Blood from some donors are fatty, after centrifugation an upper layer forms which is difficult to remove, in order to get clean plasma for cfDNA analysis. Any advice? Thanks!

01 February 2017 5,796 5 View

Can you please recommend me any free software for pie charts?

Any free software for pie charts for quality images, thank you!

11 December 2016 2,572 10 View

No title

I am wondering whether I can modify any step to increase yield, thanks a lot!!!

09 October 2016 2,579 0 View

Christmas gift!!! Can you recommend me any free software for pie charts?

Any free software for pie charts for quality images, thank you!

10 November 2015 256 1 View

How can I improve ethanol removal when precipiating DNA?

I want to remove most of ethanol following DNA precipitation, thanks!!!

06 July 2015 4,322 6 View

How can I improve DNA solubilization when using DNAzol?

How can I improve ethanol removal from final suspension?

06 July 2015 8,128 2 View

When performing immunohistochemistry and revealing with Novared, signal goes away when I dehydrate the slides. Any advice?

Data sheet from Novared (Vector) says "Prolonged incubation in alcohol or use of alcohol-based differentiating solutions may decreasesensitivity" and "Do not use recycled alcohol with this product".

31 December 2014 3,270 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View