Blood from some donors are fatty, after centrifugation an upper layer forms which is difficult to remove, in order to get clean plasma for cfDNA analysis.
Any advice? Thanks!
Domingo Miranzo-Navarro
Hi Mariana,
Hope everything is going well.
You could use a organic solvent. Here I attach a publication talking about that.
regards,
Domingo
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Christian Wechselberger
Hi Mariana,
maybe you could try piercable centrifugation tubes and simply remove your plasma-aliquot with a Syringe from beneath the fat-layer.
Best regards,
Christian
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Arvind Singh Chandel
Yes Dr Christian Wechselberger fat will separate by density gradient also
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Shaan Naughton
If you put the centrifuged plasma in a -20 freezer for 10 or so minutes the fat will solidify and you can then remove the plasma from underneath.
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