When performing cytokine ELISA, when is the best time for spleen removal?

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Marie-Ange Benoit

It depends on the cytokine you want to measure but two weeks after challenge would be a good compromise.

Mehdi Mahdavi

Hi,

When immune response diminished in response to a antigen and this time is vary base on many parameters, adjutant type, route of immunization, antigen type, dose.... but routinely 7-14 days