After adding 50% saturation of ammonium sulphate into a spent potato-dextrose liquid medium (300 mL), about 300 mg white precipitate showed up. Initially, I thought they should be proteins or lipopeptides, however, they don't have any UV absorption. I tested the solubility and found that only strong base (1 M NaOH) could dissolve them. They could be partially dissolved in 1 M HCl and not in methanol, acetone, or chloroform and ethyl acetate. How should I detect what're they? And what technique should be used for purification?
Guopeng Miao
There are no calcium in the solution, because the medium is PDA. Now I speculate that it may be starch.
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Amin Shavandi
You may check the precipitate with solid state IR to findout more on the chemical composition
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