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I am now doing EMSA with biotin linked probe, however, I can't see any unbonded free nucleotide probe. Is this normal? Should I shorten the time of electrophoresis？
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I am cloning a transporter gene of about 4.5 kb long. PCR was successful, however, when I tried to clone it into traditianal cloning vectors, such as pUC series, the target cDNA always rearranged...
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Nowadays, I am studying a gene's function using RNAi and Overexpression. The plasmids used were Gateway-based (See Figures attached). The target gene was PCR-cloned with attB site containing...
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