I get Non-specific products & multi-peak Melt Curves with my Real-Time PCR procedures, any advice on solving this problem?

I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...

11 December 2019 3,112 6 View

Can i run a single RealTime PCR experiment with 2 different Annealing Temperatures?

I have 2 different pairs of Primers, the annealing temperature of the 'reference pair' is 54C, while the temperature of the other 'test pair' is 60C. I'm running my experiment on the device of...

09 October 2019 2,002 4 View

I need an explanation for the following 8 DNA Chromatograms?

- 4 of them have a Homopolymeric Region followed by Noisy Multiple Peaks. An example image for one of the samples of this case is attached with both the sequences of its Forward and Reverse...

06 July 2018 6,834 7 View

Salting Out Method for Salivary DNA?

I use Chelex100 for extraction and MEGAquick-spinTotal for PCR purification. Some samples were very faint so i need to make salting out. Thanks in advance.

08 September 2017 6,164 4 View

PDF Protocol of Bosphore Purification Kit?

07 August 2017 8,554 2 View

Methylation Percentage of a DNA region in humans depends on number of Methylated Cytosines or Methylated Base Pairs or what ?

Thanks in advance.

09 October 2016 5,677 0 View

Any alternative for BioEdit sequence alignment editor?

07 August 2016 8,423 7 View

What is the suitable temperature for storing saliva for DNA extraction and Vitamins B test?

06 July 2016 3,238 4 View

Is the OneStep Methylation Kit suitable for DNA extracted using Chelex Extraction Kit?

05 June 2016 7,224 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View