What's the minimum purity of a genomic DNA sample used for Zymo Research's OneStep qMethyl-PCR Kit?

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I get Non-specific products & multi-peak Melt Curves with my Real-Time PCR procedures, any advice on solving this problem?

I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...

11 December 2019 3,182 6 View

Can i run a single RealTime PCR experiment with 2 different Annealing Temperatures?

I have 2 different pairs of Primers, the annealing temperature of the 'reference pair' is 54C, while the temperature of the other 'test pair' is 60C. I'm running my experiment on the device of...

09 October 2019 2,084 4 View

I need an explanation for the following 8 DNA Chromatograms?

- 4 of them have a Homopolymeric Region followed by Noisy Multiple Peaks. An example image for one of the samples of this case is attached with both the sequences of its Forward and Reverse...

06 July 2018 6,887 7 View

Salting Out Method for Salivary DNA?

I use Chelex100 for extraction and MEGAquick-spinTotal for PCR purification. Some samples were very faint so i need to make salting out. Thanks in advance.

08 September 2017 6,255 4 View

PDF Protocol of Bosphore Purification Kit?

I can't find it online, can you please send to me the PDF protocol of the kit named "Bosphore PCR Product Purification Spin Kit" of Anatolia Gene Works.

07 August 2017 8,608 2 View

Methylation Percentage of a DNA region in humans depends on number of Methylated Cytosines or Methylated Base Pairs or what ?

Thanks in advance.

09 October 2016 5,733 0 View

Any alternative for BioEdit sequence alignment editor?

BioEdit is an outdated biological sequence alignment editor: http://www.mbio.ncsu.edu/bioedit/bioedit.html Do you know alternatives for it ? Thanks in advance

07 August 2016 8,525 7 View

What is the suitable temperature for storing saliva for DNA extraction and Vitamins B test?

I am going to collect saliva from 30 persons at a remote area. This might take up to a week before returning to the lab. Can i place the samples in a freezer at a household there during that...

06 July 2016 3,304 4 View

Is the OneStep Methylation Kit suitable for DNA extracted using Chelex Extraction Kit?

Protocol of OneStep Methylation Kit: http://www.zymoresearch.com/downloads/dl/file/id/79/d5310i.pdf Protocol of Chelex DNA Extraction...

05 June 2016 7,291 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

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"A Markov-like Model for Patient Progression"?

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Why does the MFDFA algorithm need to calculate the profile of the time series?

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05 August 2024 9,366 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Ma Dejun

Maybe the protein is not completely removed. We usually get the value of 260/280nm as 1.88-2.0.

İbrahim Ertan Erkan

İf ur DNA 260/280 nm value range between 1.8-2.0 there is no problem but if it is under 1.8 , protein contamination happened. Contrary higher than 2.0 contaminated with chloroform or phenol compounds.

Best regards

Harshit Kumar

OD - 1.7 TO 1.9