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Questions related from Sarah Eissa
I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...
12 December 2019 3,172 6 View
I have 2 different pairs of Primers, the annealing temperature of the 'reference pair' is 54C, while the temperature of the other 'test pair' is 60C. I'm running my experiment on the device of...
10 October 2019 2,071 4 View
The purities of my genomic DNA samples range between 1.51 and 1.71 at 260/280nm wavelengths. This is the kit: https://www.zymoresearch.com/products/onestep-qmethyl-pcr-kit
03 March 2019 8,204 3 View
- 4 of them have a Homopolymeric Region followed by Noisy Multiple Peaks. An example image for one of the samples of this case is attached with both the sequences of its Forward and Reverse...
07 July 2018 6,880 7 View
I use Chelex100 for extraction and MEGAquick-spinTotal for PCR purification. Some samples were very faint so i need to make salting out. Thanks in advance.
09 September 2017 6,241 4 View
I can't find it online, can you please send to me the PDF protocol of the kit named "Bosphore PCR Product Purification Spin Kit" of Anatolia Gene Works.
08 August 2017 8,597 2 View
Thanks in advance.
10 October 2016 5,723 0 View
BioEdit is an outdated biological sequence alignment editor: http://www.mbio.ncsu.edu/bioedit/bioedit.html Do you know alternatives for it ? Thanks in advance
08 August 2016 8,514 7 View
I am going to collect saliva from 30 persons at a remote area. This might take up to a week before returning to the lab. Can i place the samples in a freezer at a household there during that...
07 July 2016 3,295 4 View
Protocol of OneStep Methylation Kit: http://www.zymoresearch.com/downloads/dl/file/id/79/d5310i.pdf Protocol of Chelex DNA Extraction...
06 June 2016 7,281 0 View
