I get Non-specific products & multi-peak Melt Curves with my Real-Time PCR procedures, any advice on solving this problem?

I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...

11 December 2019 3,112 6 View

Can i run a single RealTime PCR experiment with 2 different Annealing Temperatures?

I have 2 different pairs of Primers, the annealing temperature of the 'reference pair' is 54C, while the temperature of the other 'test pair' is 60C. I'm running my experiment on the device of...

09 October 2019 2,002 4 View

What's the minimum purity of a genomic DNA sample used for Zymo Research's OneStep qMethyl-PCR Kit?

The purities of my genomic DNA samples range between 1.51 and 1.71 at 260/280nm wavelengths. This is the kit: https://www.zymoresearch.com/products/onestep-qmethyl-pcr-kit

02 March 2019 8,146 3 View

I need an explanation for the following 8 DNA Chromatograms?

- 4 of them have a Homopolymeric Region followed by Noisy Multiple Peaks. An example image for one of the samples of this case is attached with both the sequences of its Forward and Reverse...

06 July 2018 6,834 7 View

Salting Out Method for Salivary DNA?

I use Chelex100 for extraction and MEGAquick-spinTotal for PCR purification. Some samples were very faint so i need to make salting out. Thanks in advance.

08 September 2017 6,164 4 View

PDF Protocol of Bosphore Purification Kit?

07 August 2017 8,554 2 View

Methylation Percentage of a DNA region in humans depends on number of Methylated Cytosines or Methylated Base Pairs or what ?

Thanks in advance.

09 October 2016 5,677 0 View

What is the suitable temperature for storing saliva for DNA extraction and Vitamins B test?

06 July 2016 3,238 4 View

Is the OneStep Methylation Kit suitable for DNA extracted using Chelex Extraction Kit?

05 June 2016 7,224 0 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View