08 August 2016 7 9K Report

BioEdit is an outdated biological sequence alignment editor: http://www.mbio.ncsu.edu/bioedit/bioedit.html

Do you know alternatives for it ? Thanks in advance

More Sarah Eissa's questions See All
I get Non-specific products & multi-peak Melt Curves with my Real-Time PCR procedures, any advice on solving this problem?

I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...

11 December 2019 3,182 6 View

Can i run a single RealTime PCR experiment with 2 different Annealing Temperatures?

I have 2 different pairs of Primers, the annealing temperature of the 'reference pair' is 54C, while the temperature of the other 'test pair' is 60C. I'm running my experiment on the device of...

09 October 2019 2,084 4 View

What's the minimum purity of a genomic DNA sample used for Zymo Research's OneStep qMethyl-PCR Kit?

The purities of my genomic DNA samples range between 1.51 and 1.71 at 260/280nm wavelengths. This is the kit: https://www.zymoresearch.com/products/onestep-qmethyl-pcr-kit

02 March 2019 8,215 3 View

I need an explanation for the following 8 DNA Chromatograms?

- 4 of them have a Homopolymeric Region followed by Noisy Multiple Peaks. An example image for one of the samples of this case is attached with both the sequences of its Forward and Reverse...

06 July 2018 6,887 7 View

Salting Out Method for Salivary DNA?

I use Chelex100 for extraction and MEGAquick-spinTotal for PCR purification. Some samples were very faint so i need to make salting out. Thanks in advance.

08 September 2017 6,255 4 View

PDF Protocol of Bosphore Purification Kit?

I can't find it online, can you please send to me the PDF protocol of the kit named "Bosphore PCR Product Purification Spin Kit" of Anatolia Gene Works.

07 August 2017 8,608 2 View

Methylation Percentage of a DNA region in humans depends on number of Methylated Cytosines or Methylated Base Pairs or what ?

Thanks in advance.

09 October 2016 5,733 0 View

What is the suitable temperature for storing saliva for DNA extraction and Vitamins B test?

I am going to collect saliva from 30 persons at a remote area. This might take up to a week before returning to the lab. Can i place the samples in a freezer at a household there during that...

06 July 2016 3,304 4 View

Is the OneStep Methylation Kit suitable for DNA extracted using Chelex Extraction Kit?

Protocol of OneStep Methylation Kit: http://www.zymoresearch.com/downloads/dl/file/id/79/d5310i.pdf Protocol of Chelex DNA Extraction...

05 June 2016 7,291 0 View

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