Protocol of OneStep Methylation Kit: http://www.zymoresearch.com/downloads/dl/file/id/79/d5310i.pdf
Protocol of Chelex DNA Extraction Kit: http://www.bio-rad.com/webroot/web/pdf/lsr/literature/LIT200.pdf
I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...
11 December 2019 3,182 6 View
I have 2 different pairs of Primers, the annealing temperature of the 'reference pair' is 54C, while the temperature of the other 'test pair' is 60C. I'm running my experiment on the device of...
09 October 2019 2,084 4 View
The purities of my genomic DNA samples range between 1.51 and 1.71 at 260/280nm wavelengths. This is the kit: https://www.zymoresearch.com/products/onestep-qmethyl-pcr-kit
02 March 2019 8,215 3 View
- 4 of them have a Homopolymeric Region followed by Noisy Multiple Peaks. An example image for one of the samples of this case is attached with both the sequences of its Forward and Reverse...
06 July 2018 6,887 7 View
I use Chelex100 for extraction and MEGAquick-spinTotal for PCR purification. Some samples were very faint so i need to make salting out. Thanks in advance.
08 September 2017 6,255 4 View
I can't find it online, can you please send to me the PDF protocol of the kit named "Bosphore PCR Product Purification Spin Kit" of Anatolia Gene Works.
07 August 2017 8,608 2 View
Thanks in advance.
09 October 2016 5,733 0 View
BioEdit is an outdated biological sequence alignment editor: http://www.mbio.ncsu.edu/bioedit/bioedit.html Do you know alternatives for it ? Thanks in advance
07 August 2016 8,525 7 View
I am going to collect saliva from 30 persons at a remote area. This might take up to a week before returning to the lab. Can i place the samples in a freezer at a household there during that...
06 July 2016 3,304 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View