Trying to isolate single cells from pancreas for FACS cell sorting. The protocole suggested 0.5mg/ml collagenase P +0.1 ng/ml DNase I per 1 ml of HBSS. Using collagenase A, the tissue wasn't digested properly after 30 min incubation @37 degree with 200 rpm agitation. Is the problem the type of collagenase or its concentration?

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