The most common method for generating drug-resistant cancer cell lines is to repeatedly treat the cells with anticancer drugs over an extended period of time.
A collection of existing drug-resistant cancer cell lines can be found here:
https://research.kent.ac.uk/industrial-biotechnology-centre/the-resistant-cancer-cell-line-rccl-collection/
Hello Dr. Samar Sami Alkafaas
The development of drug-resistant cancer cell line can take from around 3 to 18 months. If you are interested in developing resistance cancer cells in the laboratory, then you may want to refer to the article attached below.
This article summarizes the major methodological approaches for developing drug-resistant cell lines in vitro. Key decisions to be made prior to starting resistant cell line development; the choice of parent cell line, dose of selecting agent, treatment interval, and optimizing the dose of drug for the parent cell line, all of these have been discussed here.
Article In vitro Development of Chemotherapy and Targeted Therapy Dr...
However, if you wish to obtain drug resistance cancer cells, then as mentioned by Sabine Strehl , you may refer to the link above. RCCL has a collection of drug-resistance cancer cell lines from different cancer entities. The drugs used include all major classes of cytotoxic anti-cancer drugs as well as targeted agents.
Good Luck!
How I do:
1. Determine the drug IC50 for the cancer cell line.
2. Treat cells with incremental drug concentrations (IC10 to 2xIC50),each for one week.
3. Maintain a parallel culture of the parental cell line.
4. Continue culturing at 2xIC50 until cells exhibit stable growth and survival.
5. Maintain resistant cells in drug-containing media and monitor regularly.
6. Freeze aliquots of the resistant cell line at various stages for future use.
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