I am working with a polymer scaffold and I want to measure the viability of cells on it, but so far I have not been able to get good results. The general problem is that in the tests it was observed that the absorption of the scaffold without cells is higher than that of the scaffold with cells, if any of my friends who have worked in this field can help me to solve the problem Or suggest any other method to solve this issue, I will be very grateful.
Try using the WST-1 assay, which creates colored solution instead of the crystalized product in MTT. You can incubate the reagent with the cells and scaffolds first and then transfer the supernatant to a well plate for measurements.
Dear Fatemeh
Use of WST-1, CCK-8 assay kit, XTT and so on (as recommend above by dear colleagues) in which a colored solution can be analyzed instead of the crystalized products would be great. However, I suggest you first investigate the reducing property of your cell-free scaffold on MTT reagent. If the violet formazan crystals were produced by the cell-free scaffold, you should choice another group of viability assay reagents which work by different mechanism. For instance, you can measure LDH level, study DNA content, Protein content, or even use Crystal Violet assay.
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