this cell line will serve as the control group.
Thanks
That depends on the time of exposure and the doubling time your cells are experimenting, as well as the set up. If you are using common 96 well plate at 72 hours probably 10k/well is a good number, but also depends of your growing conditions.
Best you can do is a curve with different cell densities and further check which is the one that suits better for your assays
Hello Ali Gholamian Moghaddam
For MTT assay, the initial cell seeding density will depend on two factors:
1) doubling time of the cell line used and
2) the day the plate will be read (for instance, 48 hours or 72 hours).
The seeding density you decide should be such that the cells are 80-85% confluent on the day of readout. If the cells are overconfluent on the final day of the assay, the absorbance reading taken may not be as per expectation. Therefore, you need to perform the seeding density titration assay wherein you seed cells with different cell densities and select the optimum seeding density that will give you 80-85% confluency on the final day of readout.
I have attached a paper below wherein the investigators have seeded 5 × 10^3 cells/well in a 96-well culture plate for colon cells (normal (CLR-1790) as well as cancer cells) for MTT assay.
Article Differential Responses of Colorectal Cancer Cell Lines to En...
But I would recommend that you perform the seeding density titration assay and select the optimum seeding density for your experiment.
Best.
Thank you very much for your great advice. I will do that.
Best regards,
Ali
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