How to resolve the structure of a tissue engineering scaffold for immunocytochemistry testing?My scaffolding is quite dark in structure
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.
25 July 2024 7,498 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.
18 July 2024 4,218 0 View
I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.
17 July 2024 5,633 4 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...
14 July 2024 3,570 5 View
Can the kit from Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570) be used on fixed cells on a scaffold?
08 July 2024 7,914 3 View
"What is the most accurate method and model for evaluating the effective mechanical and viscoelastic properties due to the inclusion of multiple types of particles in a viscous material, and which...
16 June 2024 9,139 1 View
Hi there! We are trying to use the Concentric Circles Plugin on ImageJ to determine signal intensity in neurosphere cryosections after immunocytochemistry/confocal analysis. We determined the...
12 June 2024 7,332 0 View
hello, I have a scaffold with three layers, two synthetic polymer layers and one central layer made of ECM gel seeded with SMCs. After in vivo culture, how can I determine through Masson's...
05 May 2024 2,297 0 View
Dear connections I want to perform scaffold hopping of some molecule to get novelty on the reported compound can you suggest me the best software for the same. your support will be appreciated...
01 May 2024 5,864 1 View
can you suggest any schrodinger interface for scaffold hopping
01 May 2024 1,351 0 View
I have a reference and hit molecules. I need to compare and differentiate the scaffolds present in this hit molecules. my aim is to identify which scaffold that plays a significant role in AD.
01 May 2024 6,562 0 View
I'm performing some DAB-enhanced anti-GFAP staining. My primary is polyclonal chicken anti-GFAP (AB5541): The antibody isotype is described as N/A in the SDS. We have a goat anti-chicken IgY...
14 April 2024 9,715 1 View
I'm trying to incorporate curcumin in my polymer scaffold. 1. Is idea of soaking the 'scaffolds' in the curcumin solution is good? 2. will there not be any wastage of curcumin while...
01 April 2024 387 2 View
Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...
28 March 2024 259 3 View