What's the difference between using Collagenase P and collagenase A to dissociate mice pancreas tissue into single cells?

Trying to isolate single cells from pancreas for FACS cell sorting. The protocole suggested 0.5mg/ml collagenase P +0.1 ng/ml DNase I per 1 ml of HBSS. Using collagenase A, the tissue wasn't...

15 January 2020 9,046 2 View