13 Questions 91 Answers 0 Followers
Questions related from Reyhaneh Ravanbakhsh
Hello every one, I am looking for a tool that is able to predict if there are additional Watson-Crick pairings at the rest of sequence of microRNA. I mean after seed if still there are some...
10 October 2017 2,855 4 View
Hello everybody,I am going to cultivate MDA-MB-231 cell line along with three other cell lines. I want to know if I can cultivate this cell line with RPMI-1640 Medium under the following...
06 June 2016 7,805 18 View
06 June 2016 2,476 8 View
Hello , I'm studying on genes involved in cancer, but lately I expose with some problems. These lately studied genes have very low expression levels and I can hardly detect their expression in...
09 September 2015 1,274 10 View
Hi everyone, I am trying to extract exosomal RNA from serum using ExoQuick kit but I'm not getting any RNA from it, can anyone please tel me how to extract exosomal RNA from serum and is this kit...
01 January 2015 2,960 18 View
Hello everyoneWe are doing RT-PCR & real-time over 5-years, but during a month ago, we couldn’t get suitable results from RT-PCR, we extracted RNAs by TRYZOL kit (We already extract RNAs by...
08 August 2014 2,611 13 View
I would like to isolate a given protein as an intact and functional protein by flow cytometry (without protein unfolding or denaturation), can I do that?
04 April 2014 4,518 4 View
Can anybody please guide me whether I can use SYBR green for the product of 320bp or not? I heard that the best product size is usually less than 200bp ( 80-170 bp), but I think a 320bp product...
04 April 2014 5,031 14 View
Can anyone please share a protocol for isolation of white blood cells and extraction of RNA from these cells (RNA isolation from these cells only)?
04 April 2014 7,277 12 View
Can anybody please guide me as to how to isolate plasma for analysis of circulating microRNAs? I would like to isolate plasma immediately after blood collection (with high quality).
04 April 2014 9,181 10 View
First , sticky cells(e g. AGS cell line) were isolated using trypsin-EDTA(5mM) then cellular RNAs were extracted using RNAXplus, However, no band was observed on agarose gel! Thank you in advance...
03 March 2014 6,714 4 View
I cannot properly isolate sticky cell lines for the extraction of total RNA . I am getting low concentrations of RNA after extraction (poor bands on the agarose gel).
03 March 2014 349 4 View
I cannot properly isolate sticky cell lines for extraction of total RNA. I am getting low concentration of RNA after extraction (poor band on agarose gel).
03 March 2014 2,896 4 View
