Hi,

I can share my experience with Hippocampal tissue from rats. I used to put some crushed ice in petri dish and then use two scalpels to cut the tissue using RIPA buffer (500 ul ) until I am able to harvest the tissue using a 1 ml pipet.

I used to change the petri dish and the scalpel from one tissue to another to prevent any contamination.

There is also another way to use a plastic pestle that fits the laboratory microtubes (see the photo below) and then in ice, you can use the pestle to grind the tissue.

https://kimble-chase.com/advancedwebpage.aspx?cg=3339&cd=4&SKUTYPE=202&SKUFLD=SKU&DM=1250&WEBID=6828

hope this is helpful.

Best of LUCK

Hi

You can refer to this study. Hope this helps

• 10.1016/j.neuint.2020.104783

Hi,

I always extracted my proteins by using the pestle in the 1.5 mL eppendorfs, celllytic buffer from sigma and usually a protease inhibitor cocktail. The pestle I used was glass and was cleaned between each sample to prevent any contamination.